Ohio State junior cornerback Bradley Roby was ejected for targeting in the first quarter of the Buckeyes' home game against Iowa.
He is just the second Big Ten player ejected under the new rule; Nebraska cornerback Stanley Jean-Baptiste was ejected last week.
He was ejected after his hit on Iowa tight end C.J. Fiedorowicz.
Mike Huguenin can be reached at mike.huguenin@nfl.com. You also can follow him on Twitter @MikeHuguenin.